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KMID : 1156220050310010023
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Journal of Environmental Health Sciences
2005 Volume.31 No. 1 p.23 ~ p.30
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Detection of Staphylococcus aureus and Screening Staphylococcal Enterotoxin a, b, c genes in Strains Isolated from Strawberry Juice Shops in Jinju
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Kim Se-Ri
Shim Won-Bo
Chung Duck-Hwa
Kim Hyoung-Kab
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Abstract
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Staphylococcus aureus is one of the important pathogenic agents, which are related to the hygienic condition. This study performed for the detection of Staphylococcus aureus and screening staphylococcal enterotoxin a, b, c genes in strains isolated from the environment for production of non-pasteurized strawberry juice. A total of 44 samples were collected from utensils, machinery, employees, raw materials, and strawberry juices in 3 strawberry juice shops in Jinju, western Gyeongnam. The isolation rate of Staphylococcus aureus was 26%. Specially Staphylococcus aureus was frequently isolated from employee’s hands, strawberry and strawberry juices. The sea, seb, and sec genes were also investigated by polymerase chain reaction (PCR). One hundred and 55% of each isolate had found sea gene and seb gene, respectively. However, sec gene was not detected anywhere. To prevent food-borne disease associated with juice, the accomplishment of HACCP to be more efficient and systematic is necessary.
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KEYWORD
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